Beeswax (Cera alba) is a natural wax produced by honey bees of the genus Apis. The
wax is formed into "scales" by eight wax-producing glands in the abdominal segments 4
through 7 of worker bees, who discard it in or at the hive. The hive workers collect and use it to
form cells for honey-storage and larval and pupal protection within the beehive. Chemically, beeswax
consists mainly of esters of fatty acids and various long-chain alcohols.
Beeswax has applications in human food and flavoring, for example as a glazing agent. It is edible,
in the sense of having similar negligible toxicity to plant waxes, and is approved for food use in
the European Union under the E number E901. However, the wax monoesters in beeswax are poorly
hydrolysed in the guts of humans and other mammals, so have insignificant nutritional value.
White Beeswax BP Grade
Ph Eur
Action and use: Excipient.
DEFINITION
Wax obtained by bleaching yellow beeswax.
CHARACTERS
Appearance: White or yellowish-white pieces or plates, translucent when thin, with a
fine-grained, matt and non-crystalline fracture; when warmed in the hand they become soft and
malleable. It has an odour similar to that of yellow beeswax, though fainter and never rancid. It is
tasteless and does not stick to the teeth.
Solubility: Practically insoluble in water, partially soluble in hot ethanol (90 per cent
V/V) and completely soluble in fatty and essential oils.
Relative density: About 0.960.
TESTS
Drop point: 61C to 66C.
Melt the beeswax by heating on a water-bath, pour onto a glass plate and allow to cool to a
semi-solid mass. Fill the metal cup by inserting the wider end into the beeswax and repeating the
procedure until beeswax extrudes from the narrow opening. Remove the excess with a spatula and
insert the thermometer immediately. Remove the beeswax displaced. Allow to stand at room temperature
for at least 12 h before determining the drop point.
Acid value: 17.0 to 24.0.
To 2.00 g (m g), in a 250 ml conical flask fitted with a reflux condenser, add 40 ml of xylene and a
few glass beads. Heat until the substance is dissolved. Add 20 ml of ethanol (96 percent) and 0.5 ml
of phenolphthalein solution and titrate the hot solution with 0.5 M alcoholic potassium hydroxide
until a red colour persists for at least 10 s (n1 ml). Carry out a blank test (n2 ml).
Ester value: 70 to 80.
Saponification value: 87 to 104.
To 2.00 g (m g), in a 250 ml conical flask fitted with a reflux condenser, add 30 ml of a mixture of
equal volumes of ethanol (96 per cent) and xylene and a few glass beads. Heat until the substance is
dissolved. Add 25.0 ml of 0.5 M alcoholic potassium hydroxide and heat under a reflux condenser for
3 h. Titrate the hot solution immediately with 0.5 M hydrochloric acid , using 1 ml of
phenolphthalein solution as indicator (n1 ml). Reheat the solution to boiling several times during
the course of the titration. Carry out a blank test (n2 ml).
Ceresin, paraffins and certain other waxes: To 3.0 g, in a 100 ml round-bottomed flask, add
30 ml of a 40 g/l solution of potassium hydroxide in aldehyde-free alcohol and boil gently under a
reflux condenser for 2 h. Remove the condenser and immediately insert a thermometer. Place the flask
in a water-bath at 80C and allow to cool, swirling the solution continuously. No precipitate is
formed until 65C, although the solution may be slightly opalescent. Beginning at 65C, the solution
may become cloudy and precipitates may be formed. At 59C, the solution is cloudy.
Glycerol and other polyols: Maximum 0.5 per cent m/m, calculated as glycerol.
To 0.20 g add 10 ml of alcoholic potassium hydroxide solution and heat on a water-bath under a
reflux condenser for 30 min. Add 50 ml of dilute sulphuric acid, cool and filter. Rinse the flask
and the filter with dilute sulphuric acid. Combine the filtrate and washings and dilute to 100.0 ml
with dilute sulphuric acid. Place 1.0 ml of the solution in a test-tube, add 0.5 ml of a 10.7 g/l
solution of sodium periodate, mix and allow to stand for 5 min. Add 1.0 ml of decolorised fuchsin
solution and mix. Any precipitate disappears. Place the tube in a beaker containing water at 40C.
During cooling observe for 10-15 min. Any violet-blue colour in the solution is not more intense
than that in a standard prepared at the same time and in the same manner using 1.0 ml of a 10 mg/l
solution of glycerol R in dilute sulphuric acid.
Beeswax, White FCC Food Grade
White Wax
INS: 901
DESCRIPTION
Beeswax, White, occurs as a yellow-white solid, somewhat translucent in thin
layers, with a faint, characteristic odor, free from rancidity. It is the bleached, purified wax
from the honeycomb of the bee Apis mellifera L. (Fam. Apidae), and it consists primarily of
myricyl palmitate (myricin), cerotic acid and ester, and some high-carbon paraffins. Its specific
gravity is about 0.95. Beeswax, White, is insoluble in water and sparingly soluble in cold alcohol.
Boiling alcohol dissolves cerotic acid and part of the myricin. It is completely soluble in
chloroform, in ether, and in fixed and volatile oils. It is partly soluble in cold carbon disulfide
and is completely soluble in it at temperatures of 30C or above.
Function: Surface-finishing (glazing) agent; release agent; raw material for flavoring
agent.
REQUIREMENTS
Acid Value: Between 17 and 24.
Carnauba Wax: Passes test.
Ester Value: Between 72 and 79.
Fats, Japan Wax, Rosin, and Soap: Passes test.
Lead: Not more than 5 mg/kg.
Melting Range: Between 62° and 65°.
Saponification Cloud Test: Passes test.
Certifications :
We manufacture Bulk Drugs / API, Excipients,
Pharmaceuticals (IP/BP/USP/NF/Ph. Eur, JP/ CP), Speciality Chemicals(Pure/AR/ACS), Mineral
Fortifiers, Food Chemical Codex (FCC) and Flavour chemicals. Our manufacturing facility is
cGMP,
GLP, ISO 9001, ISO 14001, ISO 22000, ISO/IEC 17025, FSSC 22000, FSSAI, Kosher, HALAL,
EXCiPACT, WC,
COPP, WHO-GMP and WC certified. We are also REACH registered for export to European
countries.