L-Serine

General Properties and Specifications of L-Serine or (S)-2-Amino-3-hydroxypropionic acid:
Appearance: White crystalline powder.
Solubility of 10% solution 2N HCl: Clear colourless.
Assay: 99% minimum.
pH: 5 to 6 for 100 g/l in water @ 20C.
Specific rotation [α]: +12° to +14° (C=2, 5N HCl)
Sulfated Ash: 0.1% maximum.
Loss on drying: 0.5% maximum.
Chloride (CI): 0.01% maximum.
Sulfate (SO4): 0.01% maximum.
Iron (Fe): 0.001% maximum.
Heavy Metals (Pb): 0.001% maximum.
Ammonium (NH4): 0.01% maximum.
Homogeneity by TLC: Passes test.
Melting point: 222C. Decomposes.
Solubility: Soluble in water.
Storage: Room Temperature.

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Serine USP Grade Specifications:
C3H7NO3 --- 105.09
l-Serine CAS 56-45-1
UNII: 452VLY9402

DEFINITION
Serine contains NLT 98.5% and NMT 101.5% of l-serine (C3H7NO3), calculated on the dried basis.

IDENTIFICATION
A. Infrared Absorption

Residue on Ignition: NMT 0.1%
Chloride and Sulfate, Chloride: NMT 0.05%
Chloride and Sulfate, Sulfate: NMT 0.03%
Iron: NMT 30 ppm
Related Compounds: to pass the test. Individual impurities: NMT 0.5%. Total impurities: NMT 2.0%.
Optical Rotation, Specific Rotation: +14.0° to +15.6°
Loss on Drying: Dry a sample at 105C for 3 h: it loses NMT 0.2% of its weight.

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Serine BP Ph Eur Grade Specifications:
C3H7NO3 --- 105.1 CAS 56-45-1 
Action and use: Amino acid.

DEFINITION
(2S)-2-Amino-3-hydroxypropanoic acid.
Product of fermentation or of protein hydrolysis.
Content: 98.5 per cent to 101.0 per cent (dried substance).

CHARACTERS
Appearance: White or almost white, crystalline powder or colourless crystals.
Solubility: Freely soluble in water, practically insoluble in ethanol (96 per cent).

IDENTIFICATION
First identification: A, B
Second identification: A, C, D
A. Specific optical rotation.
B. Infrared absorption spectrophotometry.
C. Thin-layer chromatography.
D. To 1 mL of a 10 g/L solution of the substance to be examined in a test tube, add 5 mL of a 20 g/L solution of sodium periodate. Heat on a water-bath and collect the vapour on glass wool moistened with water and inserted in the opening of the test tube. After heating for 5 min, transfer the glass wool to a test tube containing 1 mL of a 15 g/L solution of chromotropic acid, sodium salt and 3 mL of sulfuric acid. Heat on a water-bath for 10 minutes. A violet-red colour is produced.

TESTS
Solution S: Dissolve 2.5 g in distilled water and dilute to 50 mL with the same solvent.
Appearance of solution: Solution S is clear and not more intensely coloured than reference solution6.
Specific optical rotation: + 14.0 to + 16.0 (dried substance).
Dissolve 2.50 g in dilute hydrochloric acid and dilute to 25.0 mL with the same acid.
Ninhydrin-positive substances: To pass the test.
Chlorides: Maximum 200 ppm.
Sulfates: Maximum 300 ppm.
Ammonium: To pass the test.
Iron: Maximum 10 ppm.
Loss on drying: Maximum 0.5 per cent, determined on 1.000 g by drying in an oven at 105C.
Sulfated ash: Maximum 0.1 per cent, determined on 1.0 g.

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L-Serine FCC Food Grade:
L-2-Amino-3-hydroxypropanoic Acid
C3H7NO3 Formula weight 105.09
CAS 56-45-1

DESCRIPTION
L-Serine occurs as a white crystalline powder. It is soluble in water, but is insoluble in alcohol and in ether. It melts with decomposition at about 228C.
Function: Nutrient.

REQUIREMENTS
Identification: The infrared absorption spectrum of the sample exhibits relative maxima at the same wavelengths as those of a typical spectrum as shown in the section on Infrared Spectra, using the same test conditions as specified therein.
Assay: Not less than 98.5% and not more than 101.5% of C3H7NO3, calculated on the dried basis.
Lead: Not more than 5 mg/kg.
Loss on Drying: Not more than 0.3%.
Optical (Specific) Rotation: [α]D20° Between +13.6° and +16.0°, calculated on the dried basis; or [α]D25° Between +13° and +15.6°, calculated on the dried basis.
Residue on Ignition: Not more than 0.1%.

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Certifications :
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