HEPES (4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid) has been described as one of the best all-purpose buffers available for use in biological research. HEPES is a biological buffer that functions over a pH range of 6.8 to 8.2.
General Properties and Specifications of HEPES Buffer or (N-[2-Hydroxyethyl] Piperazine-N[2-Ethane Sulfonic Acid]) or (4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid):
Appearance: White Crystalline powder
Solubility (Turbidity) 10% aq. Solution: Clear colorless
Assay: 99.5% minimum
pH (5% aq. solution): 5.0 - 6.5
Loss on drying: 0.5% maximum.
Sulphated Ash: 0.1% maximum.
Chloride (CI): 0.01% maximum.
Sulphate (SO4): 0.01% maximum.
Iron (Fe): 0.0005% maximum.
Heavy Metals (Pb): 0.0005% maximum.
Phosphate (P): 0.001% maximum.
Melting point: 234C dissociates.
Storage: Refrigerate.
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