| CAS No.: | 83-88-5 |
| Molecular Weight: | 376.36 g/mol |
| Chemical Formula | C17H20N4O6 |
Riboflavin, also known as vitamin B2, is a vitamin found in food and sold as a dietary supplement. Riboflavin or Vitamin B2 is one of the B vitamins, which are all water soluble. Riboflavin is naturally present in some foods, added to some food products, and available as a dietary supplement. In foods it has an E number of E101, E101(iii)
General Properties and Specifications of Riboflavin or Vitamin B2:
Appearance: Orange yellow crystalline powder.
Solubility of 2% solution in 0.5N NaOH: Clear orange.
Assay: 98% minimum.
Loss on drying: 1.5% maximum.
Sulfated Ash: 0.1% maximum.
pH: 5.5 - 7.2 for 0.07 g/l in water @ 20C.
Relative Density: 1.65 (Predicted).
Melting Point: 280C to 290C literature.
Boiling Point: 710C (Predicted).
Solubility: Very slightly soluble in water.
Suitability for Cell Culture: Passes.
Bacterial Endotoxin Test (BET) (EU/mg = IU/mg): <0.05 EU/mg.
Storage: Room temperature. Preferably refrigerate. In a light resistance container.
Riboflavin USP Grade Specifications:
C17H20N4O6 376.36
Riboflavine CAS 83-88-5
UNII: TLM2976OFR
DEFINITION
Riboflavin contains NLT 98.0% and NMT 102.0% of riboflavin (C17H20N4O6), calculated on the dried basis.
IDENTIFICATION
A. Color and Fluorescence of Solution:
Sample solution: 0.01 mg/mL in water
Analysis: Alternately expose to transmitted light and long-wavelength UV light.
Acceptance criteria: The Sample solution is pale greenish yellow by transmitted light. By reflected light, it exhibits an intense yellowish-green fluorescence that disappears upon the addition of mineral acids or alkalies.
Residue on Ignition: NMT 0.3%
Limit of Lumiflavin: To pass the test.
Optical Rotation, Specific Rotation:
Sample solution: 5 mg/mL in 0.05 M carbonate-free sodium hydroxide.
Analysis: Measure the specific rotation within 30 min of preparation.
Acceptance criteria: −115° to −135°
Loss on Drying: Dry 500 mg at 105C for 2 h. It loses NMT 1.5% of its weight.
Riboflavin BP Ph Eur Grade Specifications:
C17H20N4O6 --- 376.4 CAS 83-88-5
Action and use: Vitamin B2.
DEFINITION
7,8-Dimethyl-10-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]benzo[g]pteridine-2,4(3H,10H)-dione.
This monograph applies to riboflavin produced by fermentation.
Content: 97.0 per cent to 103.0 per cent (dried substance).
CHARACTERS
Appearance: Yellow or orange-yellow, crystalline powder.
Solubility: Very slightly soluble in water, practically insoluble in ethanol (96 per cent).
Solutions deteriorate on exposure to light, especially in the presence of alkali.It shows polymorphism.
IDENTIFICATION
A. Specific optical rotation.
B. Thin-layer chromatography.
C. Dissolve about 1 mg in 100 mL of water. The solution has, by transmitted light, a pale greenish yellow colour, and, by reflected light, an intense yellowish-green fluorescence which disappears on the addition of mineral acids or alkalis.
Specific optical rotation: -115 to -135 (dried substance).
Dissolve 50.0 mg in 0.05 M sodium hydroxide free from carbonate and dilute to 10.0 mL with the same alkaline solution. Measure the optical rotation within 30 min of dissolution.
Absorbance:
Test solution Dilute the final solution prepared for the assay with an equal volume of water.
Absorption maxima at 223 nm, 267 nm, 373 nm and 444 nm.
Absorbance ratios:
— A373/A267 = 0.31 to 0.33;
— A444/A267 = 0.36 to 0.39.
Related substances: To pass the test.
Loss on drying: Maximum 1.5 per cent, determined on 1.000 g by drying in an oven at 105C.
Sulfated ash: Maximum 0.1 per cent, determined on the residue obtained in the test for loss on drying.
Riboflavin FCC Food Grade Specifications:
Vitamin B2
C17H20N4O6 Formula weight 376.37
INS: 101(i) CAS 83-88-5
DESCRIPTION
Riboflavin occurs as a yellow to orange-yellow, crystalline powder. When dry, it is not affected by diffused light, but when in solution, light induces deterioration. It melts at about 280° with decomposition, and its saturated solution is neutral to litmus. One-gram dissolves in 3000 to about 20,000 mL of water, the variations being due to differences in the internal crystalline structure. It is less soluble in alcohol than in water. It is insoluble in ether and in chloroform, but it is very soluble in dilute solutions of alkalis.
Function: Nutrient.
