| CAS No.: | 657-27-2 |
| Molecular Weight: | 182.65 g/mol |
| Chemical Formula | C6H14N2O2-HCl or H2N(CH2)4CH(NH2)CO2H-HCl |
Packaging and storage: Preserve in well-closed containers.
Identification: Infrared Absorption.
Specific rotation: between +20.4 and +21.4 .
Loss on drying: Dry it at 105C for 3 hours: it loses not more than 0.4% of its weight.
Residue on ignition: not more than 0.1%.
Sulfate: A solution containing 0.33 g shows no more sulfate than corresponds to 0.10 mL of 0.020 N sulfuric acid (0.03%).
Iron: 0.003%.
Heavy metals: 0.0015%.
Chromatographic purity: To pass the test.
Content of chloride: Transfer about 350 mg, accurately weighed, to a porcelain casserole, and add 140 mL of water and 1 mL of dichlorofluorescein. Mix, and titrate with 0.1 N silver nitrate until the silver chloride flocculates and the mixture acquires a faint pink color. Each mL of 0.1 N silver nitrate is equivalent to 3.545 mg of chloride: between 19.0% and 19.6% is found.
Assay: Transfer about 90 mg of Lysine Hydrochloride, accurately weighed, to a 125-mL flask, and dissolve in a mixture of 3 mL of formic acid and 50 mL of glacial acetic acid. Add 10 mL of mercuric acetate and titrate with 0.1 N perchloric acid, determining the endpoint potentiometrically. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 9.133 mg of C6H14N2O2.HCl.
Lysine Hydrochloride BP Ph Eur Grade
C6H14N2O2-HCl --- 182.7 --- CAS 657-27-2
Action and use: Amino acid.
DEFINITION
Lysine hydrochloride contains not less than 98.5 per cent and not more than the equivalent of 101.0 per cent of (S)-2,6-diaminohexanoic acid hydrochloride, calculated with reference to the dried substance.
CHARACTERS
A white or almost white, crystalline powder or colourless crystals, freely soluble in water, slightly soluble in alcohol.
IDENTIFICATION
First identification: A, B, E.
Second identification: A, C, D, E.
A. It complies with the test for specific optical rotation.
B. Examine by infrared absorption spectrophotometry, comparing with the spectrum obtained with lysine hydrochloride CRS. Examine the substances prepared as discs. If the spectra obtained show differences, dissolve the substance to be examined and the reference substance separately in the minimum volume of water, evaporate to dryness at 60C, and record new spectra using the residues.
C. Examine the chromatograms obtained in the test for ninhydrin-positive substances. The principal spot in the chromatogram obtained with test solution (b) is similar in position, colour and size to the principal spot in the chromatogram obtained with reference solution (a).
D. To 0.1 ml of solution S (see Tests) add 2 ml of water and 1 ml of a 50 g/l solution of phosphomolybdic acid. A yellowish-white precipitate is formed.
E. To 0.1 ml of solution S add 2 ml of water. The solution gives reaction (a) of chlorides.
TESTS
Solution S: Dissolve 5.0 g in carbon dioxide-free water R prepared from distilled water and dilute to 50 ml with the same solvent.
Appearance of solution: Solution S is clear and not more intensely coloured than reference solution.
Specific optical rotation: Dissolve 2.00 g in hydrochloric acid and dilute to 25.0 ml with the same acid. The specific optical rotation is + 21.0 to + 22.5, calculated with reference to the dried substance.
Ninhydrin-positive substances: To pass the test.
Sulphates: Dilute 5 ml of solution S to 15 ml with distilled water R. The solution complies with the limit test for sulphates (300 ppm).
Ammonium: 50 mg complies with limit test B for ammonium (200 ppm). Prepare the standard using 0.1 ml of ammonium standard solution (100 ppm NH4).
Iron: In a separating funnel, dissolve 0.33 g in 10 ml of dilute hydrochloric acid. Shake with three quantities, each of 10 ml, of methyl isobutyl ketone, shaking for 3 min each time. To the combined organic layers add 10 ml of water and shake for 3 min. The aqueous layer complies with the limit test for iron (30 ppm).
Heavy metals: 12 ml of solution S complies with limit test A for heavy metals (10 ppm).
Loss on drying: Not more than 0.5 per cent, determined on 1.000 g by drying in an oven at 105C.
Sulphated ash: Not more than 0.1 per cent, determined on 1.0 g.
ASSAY
Dissolve 0.150 g in 5 ml of anhydrous formic acid. Add 50 ml of anhydrous acetic acid. Titrate with 0.1 M perchloric acid, determining the end-point potentiometrically. 1 ml of 0.1 M perchloric acid is equivalent to 18.27 mg of C6H15ClN2O2.
STORAGE
Store protected from light.
L-Lysine Monohydrochloride FCC Food Grade
2,6-Diaminohexanoic Acid Hydrochloride
C6H14N2O2-HCl --- Formula wt 182.65 --- CAS: [657-27-2]
DESCRIPTION
L-Lysine Monohydrochloride occurs as a white or nearly white, free-flowing, crystalline powder. It is freely soluble in water, but it is almost insoluble in alcohol and in ether. It melts at about 260° with decomposition.
Function: Nutrient.
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