| CAS No.: | 61-90-5 |
| Molecular Weight: | 131.17 g/mol |
| Chemical Formula | C6H13NO2 |
L-Leucine or L-2-Amino-4-methylvaleric Acid or (2S)-2-Amino-4-methylpentanoic Acid is an essential amino acid, which can be used in nutritional supplements and generally used for bread, bread products. Used as a food additive, L-leucine has E number E641 and is classified as a flavor enhancer.
General Properties and Specifications of L-Leucine or L-2-Amino-4-methylvaleric Acid or (2S)-2-Amino-4-methylpentanoic Acid:
Appearance: White crystalline powder.
Solubility of 10% solution 2N HCl: Clear colourless.
pH (1% aq. solution): 5.5 to 7.0
Assay: 99% minimum.
Loss on drying: 0.5% maximum.
Sulfated Ash: 0.1% maximum.
Chloride (CI): 0.02% maximum.
Sulfate (SO4): 0.01% maximum.
Iron (Fe): 0.001% maximum.
Heavy Metals (Pb): 0.001% maximum.
Ammonium (NH4): 0.02% maximum.
Homogeneity by TLC: Passes test.
Leucine USP Grade Specifications:
C6H13NO2 --- 131.17
l-Leucine CAS 61-90-5
UNII: GMW67QNF9C
DEFINITION
Leucine contains NLT 98.5% and NMT 101.5% of l-leucine (C6H13NO2), calculated on the dried basis.
IDENTIFICATION
A. Infrared Absorption.
Residue on Ignition: NMT 0.4%
Chloride and Sulfate, Chloride: NMT 0.05%
Chloride and Sulfate, Sulfate: NMT 0.03%
Iron: NMT 30 ppm
Related Compounds: To pass the test.
Optical Rotation, Specific Rotation:
Sample solution: 40 mg/mL in 6 N hydrochloric acid.
Acceptance criteria: +14.9° to +17.3°
pH: 5.5–7.0
Loss on Drying: Dry at 105c for 3 h.
Acceptance criteria: NMT 0.2%
Leucine BP Ph Eur Grade Specifications:
C6H13NO2 --- 131.2 CAS 61-90-5
Action and use: Amino acid.
DEFINITION
(2S)-2-Amino-4-methylpentanoic acid.
Product of fermentation or of protein hydrolysis.
Content: 98.5 per cent to 101.0 per cent (dried substance).
CHARACTERS
Appearance: White or almost white, crystalline powder or shiny flakes.
Solubility: Sparingly soluble in water, practically insoluble in ethanol (96 per cent). It dissolves in dilute mineral acids and in dilute solutions of alkali hydroxides.
IDENTIFICATION
First identification: A, B
Second identification: A, C
A. Specific optical rotation.
B. Infrared absorption spectrophotometry.
C. Thin-layer chromatography.
TESTS
Appearance of solution: The solution is clear and not more intensely coloured than reference solution.
Specific optical rotation: + 14.5 to + 16.5 (dried substance).
Dissolve 1.00 g in hydrochloric acid and dilute to 25.0 mL with the same acid.
Ninhydrin-positive substances: To pass the test.
Chlorides: Maximum 200 ppm.
Sulfates: Maximum 300 ppm.
Ammonium: To pass the test.
Iron: Maximum 10 ppm.
Loss on drying: Maximum 0.5 per cent, determined on 1.000 g by drying in an oven at 105 °C.
Sulfated ash: Maximum 0.1 per cent, determined on 1.0 g.
L-Leucine FCC Food Grade Specifications:
L-2-Amino-4-methylvaleric Acid
C6H13NO2 Formula weight 131.17
INS: 641 CAS: 61-90-5
DESCRIPTION
L-Leucine occurs as small, white, lustrous plates, or as a white, crystalline powder. One-gram dissolves in about 40 mL of water and in about 100 mL of glacial acetic acid. It is sparingly soluble in alcohol, and soluble in dilute hydrochloric acid and in solutions of alkali hydroxides and carbonates.
Function: Nutrient.
