| CAS No.: | 73-32-5 |
| Molecular Weight: | 131.17 g/mol |
| Chemical Formula | C6H13NO2 |
L-Isoleucine or (2S,3S)-2-Amino-3-methylpentanoic acid is an essential amino acid used as a supplement in sports nutrition. L-Isoleucine is also used as a feed additive to meet the requirements of target animals like Cattle, Chicken, Dog, Horse, Pigs, and fish.
General Properties and Specifications of L-Isoleucine or (2S,3S)-2-Amino-3-methylpentanoic acid:Isoleucine USP Grade Specifications:
C6H13NO2 131.17
l-Isoleucine CAS 73-32-5
UNII: 04Y7590D77
DEFINITION
Isoleucine contains NLT 98.5% and NMT 101.5% of l-isoleucine (C6H13NO2), calculated on the dried basis.
IDENTIFICATION
A. Infrared Absorption
Residue on Ignition: NMT 0.3%
Chloride and Sulfate, Chloride: Acceptance criteria: NMT 0.05%
Chloride and Sulfate, Sulfate: Acceptance criteria: NMT 0.03%
Iron: NMT 30 ppm.
Related Compounds: To pass the test.
Optical Rotation, Specific Rotation: 40 mg/mL in 6 N hydrochloric acid
Acceptance criteria: +38.9° to +41.8°
pH: 5.5–7.0 in 10 mg/mL in water.
Loss on Drying at 105C for 3 h: NMT 0.3%
Isoleucine BP Ph Eur Grade Specifications:
C6H13NO2 --- 131.2 CAS 73-32-5
Action and use: Amino acid.
DEFINITION
(2S,3S)-2-Amino-3-methylpentanoic acid.
Product of fermentation or of protein hydrolysis.
Content: 98.5 per cent to 101.0 per cent (dried substance).
CHARACTERS
Appearance: White or almost white, crystalline powder or flakes.
Solubility: Sparingly soluble in water, slightly soluble in ethanol (96 per cent). It dissolves in dilute mineral acids and in dilute solutions of alkali hydroxides.
IDENTIFICATION
First identification: A, B
Second identification: A, C
A. Specific optical rotation.
B. Infrared absorption spectrophotometry.
C. Thin-layer chromatography.
