| CAS No.: | 56-86-0 |
| Molecular Weight: | 147.13 g/mol |
| Chemical Formula | HO2CCH2CH2CH(NH2)CO2H |
Specifications of Glutamic Acid USP Dietary Supplement:
C5H9NO4 --- 147.13
l-Glutamic acid;
S-2-Aminopentanedioic acid --- CAS 56-86-0.
UNII: 3KX376GY7L
DEFINITION
Glutamic Acid contains NLT 98.5% and NMT 101.5% of l-glutamic acid (C5H9NO4), calculated on the dried basis.
IDENTIFICATION
Infrared Absorption 〈197K〉.
ASSAY
To pass the test.
Acceptance criteria: 98.5%–101.5% on the dried basis.
Residue on Ignition: NMT 0.1%.
Chloride and Sulfate, Chloride:
To pass the test.
Acceptance criteria: NMT 0.02%
Chloride and Sulfate, Sulfate:
To pass the test.
Acceptance criteria: NMT 0.02%
Iron: NMT 10 µg/g
Related Compounds:
To pass the test.
Acceptance criteria: Any secondary spot of the Sample solution is not larger or more intense than the principal spot of the Standard solution.
Individual impurities: NMT 0.5%
Total impurities: NMT 2.0%
Optical Rotation, Specific Rotation:
To pass the test.
Acceptance criteria: +31.5° to +32.5°
Loss on Drying: Dry a sample at 105C for 3 h: it loses NMT 0.1% of its weight.
Packaging and Storage: Preserve in well-closed containers, and store at controlled room temperature.
Specifications of Glutamic Acid BP Ph Eur Grade:
C5H9NO4 --- 147.1 --- 56-86-0
Action and use: Amino acid.
DEFINITION
Glutamic acid contains not less than 98.5 per cent and not more than the equivalent of 100.5 per cent of (2S)-2-aminopentanedioic acid, calculated with reference to the dried substance.
CHARACTERS
A white or almost white, crystalline powder or colourless crystals, freely soluble in boiling water, slightly soluble in cold water, practically insoluble in acetic acid, in acetone and in alcohol.
IDENTIFICATION
First identification: A, B
Second identification: A, C, D
A. Specific optical rotation.
B. Examine by infrared absorption spectrophotometry, comparing with the spectrum obtained with glutamic acid CRS. Examine the substances prepared as discs. If the spectra obtained show differences, dissolve the substance to be examined and the reference substance separately in the minimum quantity of water, evaporate to dryness at 60C and record new spectra using the residues.
C. Examine the chromatograms obtained in the test for ninhydrin-positive substances. The principal spot in the chromatogram obtained with test solution (b) is similar in position, colour and size to the principal spot in the chromatogram obtained with reference solution (a).
D. To 2.0 mL of solution S (see Tests) add 0.1 mL of phenolphthalein solution and 3.0 mL to 3.5 mL of1 M sodium hydroxide to change the colour of the indicator to red. Add a mixture of 3 mL of formaldehyde solution, 3 mL of carbon dioxide-free water and 0.1 mL of phenolphthalein solution, to which sufficient1 M sodium hydroxide has been added to produce a pink colour. The solution is decolourised. Add1 M sodium hydroxide until a red colour is produced. The total volume of 1 M sodium hydroxide used is 4.0 mL to 4.7 mL.
TESTS
Solution S: Dissolve 5.00 g in 1 M hydrochloric acid with gentle heating and dilute to 50.0 mL with the same acid.
Appearance of solution: Solution S is clear and colourless.
Specific optical rotation: + 30.5 to + 32.5, determined on solution S and calculated with reference to the dried substance.
Ninhydrin-positive substances: To pass the test.
Chlorides: Dissolve 0.25 g in 3 mL of dilute nitric acid and dilute to 15 mL with water. The solution, to which 1 mL of water is added instead of dilute nitric acid, complies with the limit test for chlorides (200 ppm).
Sulfates: Dilute 5 mL of solution S to 15 mL with distilled water. The solution complies with the limit test for sulfates (300 ppm).
Ammonium: 50 mg complies with limit test B for ammonium (200 ppm). Prepare the standard using 0.1 mL of ammonium standard solution (100 ppm NH4).
Iron: In a separating funnel, dissolve 1.0 g in 10 mL of dilute hydrochloric acid. Shake with 3 quantities, each of 10 mL, of methyl isobutyl ketone, shaking for 3 min each time. To the combined organic layers add 10 mL of water and shake for 3 min. The aqueous layer complies with the limit test for iron (10 ppm).
Loss on drying: Not more than 0.5 per cent, determined on 1.000 g by drying in an oven at 105C.
Sulfated ash: Not more than 0.1 per cent, determined on 1.0 g.
ASSAY
Dissolve 0.130 g in 50 mL of carbon dioxide-free water with gentle heating. Cool. Using 0.1 mL of bromothymol blue solution as indicator, titrate with 0.1 M sodium hydroxide until the colour changes from yellow to blue.
1 mL of 0.1 M sodium hydroxide is equivalent to 14.71 mg of C5H9NO4.
STORAGE
Protected from light.
Specifications of L-Glutamic Acid FCC Food Grade:
Glutamic Acid; L-2-Aminopentanedioic Acid
C5H9NO4 Formula weight 147.13
INS: 620 CAS: 56-86-0
DESCRIPTION
L-Glutamic Acid occurs as a white, free-flowing, crystalline powder. It is slightly soluble in water, forming acidic solutions. The pH of a saturated solution is about 3.2.
Function: Salt substitute; nutrient.
