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L-Alanine

CAS No.: 56-41-7
Molecular Weight: 89.09 g/mol
Chemical Formula C3H7NO2

Alanine is an amino acid commonly found as a component of total parenteral nutrition. Alanine or L-alpha-alanine or D-alpha-alanine is a non-essential amino acid. It is an important source of energy for muscle tissue, the brain and central nervous system; strengthens the immune system by producing antibodies; helps in the metabolism of sugars and organic acids.

General Properties and specifications of L-Alanine or (S)-2-Aminopropionic Acid or L-α-Aminopropionic Acid:
Appearance: White crystalline powder.
Assay: 99%
pH: 5.5 - 7.0 for 89.1 g/l in water @ 25C.
Relative density: around 1.43
Melting point: 314C (decomposes).
Solubility: Soluble in water.


Alanine USP Grade Specifications:
C3H7NO2 89.09
l-Alanine CAS 56-41-7
UNII: OF5P57N2ZX

DEFINITION
Alanine contains NLT 98.5% and NMT 101.5% of l-alanine (C3H7NO2), calculated on the dried basis.

IDENTIFICATION
A. Infrared Absorption.
Residue on Ignition: NMT 0.15%
Chloride and Sulfate, Chloride: NMT 0.05%
Chloride and Sulfate, Sulfate: NMT 0.03%
Iron: NMT 30 ppm.
Related Compounds: To pass the test by chromatography.
Optical Rotation, Specific Rotation: +13.7° to +15.1°
pH: 5.5–7.0, in a solution (1 in 20).
Loss on Drying: NMT 0.2% at 105C.


Alanine BP Ph Eur Grade Specifications:
C3H7NO2 --- 89.1 CAS 56-41-7
Action and use: Amino acid.

DEFINITION
(2S)-2-Aminopropanoic acid.
Content: 98.5 per cent to 101.0 per cent (dried substance).

CHARACTERS
Appearance: White or almost white, crystalline powder or colourless crystals.
Solubility: Freely soluble in water, very slightly soluble in ethanol (96 per cent).

IDENTIFICATION
First identification: A, B
Second identification: A, C, D
A. Specific optical rotation.
B. Infrared absorption spectrophotometry.
C. Thin-layer chromatography.
D. Dissolve 0.5 g in a mixture of 0.25 mL of hydrochloric acid, 0.5 mL of a 100 g/L solution of sodium nitrite and 1 mL of water. Shake; gas is given off. Add 2 mL of dilute sodium hydroxide solution, followed by 0.25 mL of iodinated potassium iodide solution. After about 30 min, a yellow precipitate is formed.

TESTS
Solution S: Dissolve 2.5 g in distilled water and dilute to 50 mL with the same solvent.
Appearance of solution: The solution is clear and not more intensely coloured than reference solution.
Specific optical rotation: + 13.5 to + 15.5 (dried substance).
Ninhydrin-positive substances: To pass the test.
Chlorides: Maximum 200 ppm.
Sulfates: Maximum 300 ppm.
Ammonium: To pass the test.
Iron: Maximum 10 ppm.
Loss on drying: Maximum 0.5 per cent, determined on 1.000 g by drying in an oven at 105C.
Sulfated ash: Maximum 0.1 per cent, determined on 1.0 g.


L-Alanine FCC Food Grade Specifications:
L-2-Aminopropanoic Acid
C3H7NO2 Formula weight 89.09
CAS 56-41-7

DESCRIPTION
L-Alanine occurs as a white crystalline powder. It is freely soluble in water, sparingly soluble in alcohol, and insoluble in ether. The pH of a 1:20 aqueous solution is between 5.5 and 7.0.
Function; Nutrient.

REQUIREMENTS
Identification: The infrared absorption spectrum of the sample exhibits relative maxima at the same wavelengths as those of a typical spectrum as shown in the section on Infrared Spectra, using the same test conditions as specified therein.
Assay: Not less than 98.5% and not more than 101.5% of C3H7NO2, calculated on the dried basis.
Lead: Not more than 5 mg/kg.
Loss on Drying: Not more than 0.3%.
Optical (Specific) Rotation: [α]D20°: Between +13.5° and +15.5°, calculated on the dried basis; or [α]D25°: between +13.2° and +15.2°, calculated on the dried basis.
Residue on Ignition: Not more than 0.2%.